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KMID : 0380219940270040316
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 4 p.316 ~ p.322
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Stimulation of Poly(3-hydroxybutyrate) Degradation by Nitrogen Nutrient in Alcaligenes eutorphus H16
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Abstract
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Abstract:
@EN Poly(3-hydroxybutyrate) (PHB) accumulated in Alcaligenes eutrophus H16 when nitrogen nutrient was limited. The addition of nitrogen nutrient (NH4Cl) to the medium during PHB synthesis caused the bacteria to cease PHB accumulation, indicating
that
the synthesis of PHB is regulated by nitrogen assimilation. Accumulated PHB increased when bacteria were transferred to carbon-free media, even in the absence of nitrogen. However, the degradation of PHB in cells was stimulated by the addition of
nitrogen nutrient to carbon-free media. The activity of intracellular PHB depolymerase was measured using 14C-labeled PHB as a substrate. While autodegradation of PHb native granules occurred in vitro, little enzyme activity was detected in the
cytosolic fraction, suggesting that most of the enzyme is bound to PHB granules. Autodegradation in the cytosolic fraction, suggesting that most of the enzyme is bound to PHB granules. Autodegradation of PHB was detected at two different pH
values,
7.0
and 10.5. Although the effects of inhibitors, such as phenylmethylsulfonyl fluoride and Triton X-100, on autodegradation were similar at both pH values, the effects of dithiothreitol, MgCI2, and bovine and bovine serum albumin were different
depending
on pH.
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